Molecular detection and analysis of Y. pestis
نویسنده
چکیده
The polymerase chain reaction (PCR) has been used to detect Y. pestis in infected fleas, soil samples, and clinical samples for rapid diagnosis of plague. PCR-gradient density electrophoresis (PCR-GDE) has allowed the distinction between the three pathogenic Yersinia species utilizing the rpoB gene. The application of the PCR to dental samples has allowed the confirmation of Y. pestis as the cause of death in archaeological samples dating to 600 AD. In addition, real-time PCR (Rti-PCR) methodology has also been developed for detection of Y. pestis. Target sequences have included unique chromosomal sequences specific for Y. pestis in addition to genes specific for Y. pestis including pla, caf1, Pst. The use of variablenumber tandem repeats (VNTRs) has allowed the mutation rate of Y. pestis to be determined in addition to distinguishing different strains of Y. pestis and the three major biovars. PCR primers have also been developed for confirming the presence of each of the three major virulence plasmids in Y. pestis.
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